The laboratory part of the Scanning Electron Microscopy Course Bio G 4 01, Transmission Electron Microscopy Course Bio G 403, and Freeze-Fracture Course Bio G 606 are offered at the CIMC. The courses are recommended also to those who intend to use the CIMC electron microscopes and have had no prior training. Individual instructions on a fee basis are also offered at the CIMC. Details can be obtained by sending an E-Mail to: CIMC-mailbox@cornell.edu. BIO G 401 Course Page BIO G 401 Introduction to Scanning Electron Microscopy . Fall or spring, weeks 1-8. 1 credit. S-U grades optional. Lec, M 10:10; lab TR or F 9:05-12:15 or TW or R 1:25-4:25. M.V. Parthasarathy. An introductory course that includes the principle and the proper use of the scanning electron microscope. Students use biological material to explore and interpret some of the fine biological architecture observed. In addition to preparing various specimens, the students will use the scanning electron microscope to study and obtain micrographs of features in the samples that interest them. A LEO DSM 960 Digital Scanning Electron Microscope (SEM), a BAL-TEC Sputter Coater, and a BAL-TEC Critical Point Dryer would be used for the course. A high resolution laser printer will be used to print the SEM digital images saved through a computer using an image grabber. BIO G 403 Course Page BIO G 403 Transmission Electron Microscopy for Biologists Fall. 1, 3, or 4 credits (4 credits if student takes both sections). Limited to 12 students. Prerequisites: BIOAP 313, BIOPL 345 or 443, or permission of instructor. S-U grades optional. Lec, T 11:15; labs, M W or TR 1:25-4:25. Two sections: Sec 01, 1 credit, weeks 1-4; sec 02, 3 credits, weeks 5-12. Students may register for one or both sections. M.V. Parthasarathy Section 01, 1 credit, weeks 1-4, covers the principles and use of the transmission electron microscopy (TEM), with emphasis on proper preparation of the instrument and interpretation of images obtained. Negatively stained materials are used for viewing with the transmission electron microscopy. Section 02, 3 credits, weeks 5-12, covers the principles and techniques of preparing biological material for transmission electron microscopy. Using a wide range of biological materials this section studies chemical fixation/embedding, ultrathin sectioning, immunogold localization, and quantitative microscopy techniques. Students have two additional weeks to complete laboratory assignments at the end of each section. A Philips EM 201 Transmission electron microscope, and two Reichert Ultramicrotomes would be used for the course. Images for Section 1 would be obtained using photographic negatives and prints. For Section 2, photographic negatives of the TEM images will be digitized through a scanner and the digitized images will be printed using a high resolution laser printer. Students can choose one of the specimens provided for their concentration. BIO G 606 Freeze-Fracture Technique Spring, weeks 9-14. 1 credit. Primarily for graduate students. Limited to 8 students. Prerequisites: BIO G 403 or equivalent, and permission of instructor. S-U grades only. Lec, M 10:10; disc to be arranged; labs, MW 1:25-4:25. M.V.Parthasarathy. Principles of rapid freezing and freeze-fracture techniques, freezing artifacts, and interpretation of images. Unicellular organisms would be used for the course. Light Microscopy Informal training will be offered periodically for the use of the ZEISS Axioplan2 and the software associated with it for imaging, including Digital Confocal Microscopy. See our gallery of micrographs for examples, and our web page http://www.cimc.cornell.edu/Pages/resources.htm to find out about the light microscopy equipment. Depending on the trainees' experience, the training session can last from 6 to 12 hrs. The first 6 hrs of training is free of charge. Please contact us at cimc-mailbox@cornell.edu for the next informal training date. See our web site page http://www.cimc.cornell.edu/Pages/fee.htm for the fee schedules.

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