Fee Schedule for Cornell University Projects Cornell University users and projects can take advantage of the services offered at CIMC on the fee basis shown below. Users outside of the Cornell community should inquire about service fees by contacting the center by e-mail at cimc-mailbox@cornell.edu. Transmission Electron Microscopy Services SAMPLE PREPARATION Negative Stain Preparation of grids = $35/hour Formvar / carbon coated grids = $2/grid Standard Thin Sections Chemical Fixation, Dehydration & Embedding (21 blocks maximum) (choose one option) Routine Samples: 1st sample = $110 after 1st sample (submitted at the same time) = $30 ea. Cell Culture or Particulates: 1st sample = $175 after 1st sample (submitted at the same time) = $40 ea. Sectioning Thick sections = $12 / block Thin sectioning & staining = $40 / block (minimum of 6 grids) Additional thin sections OR sections of specific area of interest = $40 / hour Thin Sections with Immuno-gold Labeling Chemical Fixation, Dehydration & Embedding (21 blocks maximum) (choose one option) Routine Samples: 1st sample = $110 after 1st sample (submitted at the same time) = $30 ea. Cell Culture or Particulates: 1st sample = $175 after 1st sample (submitted at the same time) = $40 ea. Sectioning: Thick sections = $12 / block Thin sectioning + staining = $75 / block (minimum of 6 grids) Extra grids = $75 for 10 grids Cryo Fixation with Standard Sectioning High Pressure Freezing with Technical help & supplies = $200 / day Freeze Substitution: 1st sample = $175 after 1st sample (submitted at the same time) = $60 ea. Sectioning: Thicks = $12 / block Thin sectioning & staining = $40 / block (minimum of 6 grids) Additional thin sections OR sections of specific area of interest = $40 / hour Cryo Fixation with Cryo Sectioning (NOTE: This service not currently available.) Plunge Freezing = hourly @ $35 / hour Sections = hourly @ $40 / hour Consumables (LN2) = $40 / day Freeze Fracture With Technical help & supplies = $80 / run MICROSCOPE USE Standard viewing = $65 / hour Cryo stage (for use with cryo sections only) = $?? / hour (NOTE: This service not currently available.) IMAGING Digital images to send/preview via email = $2 ea. TEM negatives = $0.95 ea. Negative Processing = hourly @ $35 / hour Publication quality 5x7 prints of TEM negative = $8; additional prints of same negative = $3 ea. Scanning Electron Microscopy Services SAMPLE PREPARATION Standard Mount of Dry / Hard Specimen Mounting of samples on stubs = hourly @ $35 / hour Sputter Coating = $15 / run Chemical Fixation Chemical Fixation & Dehydration (choose one option) Routine Samples: 1st sample = $60 after 1st sample (submitted at the same time) = $30 ea. Cell Culture or Particulates: 1st sample = $85 after 1st sample (submitted at the same time) = $40 ea. Critical Point Drying = $35 / run for Tech. help + $10 / run for consumables Mounting of samples on stubs = hourly @ $35 / hour Sputter Coating = $15 / run Cryo Fixation for Standard Viewing Plunge Freezing = hourly @ $35 / hour Freeze Drying = $5 / day Mounting of samples on stubs = hourly @ $35 / hour Sputter Coating = $15 / run Cryo Fixation for Use with Cryostage (choose one of two freezing options) Plunge Freezing = hourly @ $35 / hour High Pressure Freezing with Technical help & supplies = $200 / day MICROSCOPE USE Standard viewing = $65 / hour X-ray analysis of samples (optional) = $70 / hour Cryostage operation with Technical help & supplies (for use with method "D" above) = $375 / day IMAGING Digital images to send/preview via email = $2 ea. Video Prints = $0.25 ea. SEM Polaroids (4"x5") Type 55 = $3 ea. Publication quality 5x7 prints of SEM negative = $8; additional prints of same negative = $3 ea. LIGHT MICROSCOPY SERVICES SAMPLE PREPARATION Wet Preparations Wet preparations = $35 / hour Standard Thick Sections Chemical Fixation, Dehydration & Embedding (21 blocks maximum) (choose one option) Routine Samples: 1st sample = $110 after 1st sample (submitted at the same time) = $30 ea. Cell Culture or Particulates: 1st sample = $175 after 1st sample (submitted at the same time) = $40 ea. Sectioning: Thick sections = $20 / block for 1.0 um sections Additional thick sections OR sections of specific area of interest = $40 / hour MICROSCOPE USE Standard viewing = $35 / hour IMAGING Customers should provide their own zip disk for image storage. Training of Users: Training for using the equipment in the facility may be obtained through regular E.M. courses (Fall: BioG 403 for T.E.M., BioG 401 for S.E.M., Spring: BioG 401 for S.E.M.). Individual training is only occasionally available on an hourly fee basis as time allows. Training on ancillary equipment such as ultramicrotome, coaters, etc. will involve additional time. TEM Ultramicrotome $5 / hour Cryo-ultramicrotome $60 / day supplies for Cryo-ultramicrotome $40 / day Freeze Fracture $30 / run T.E.M. (Philips 201) $25 / hour T.E.M. (Philips Tecnai 12 Biotwin) $30 / hour Electron Microscopy Film $0.95 / ea. Photographic darkroom equipment and chemicals $5 / hour      * NOTE: photographic paper for printing is NOT provided   SEM Critical Point Dryer $ 10 / run Freeze-dryer $ 5 / day Sputter Coater $ 10 / run Thermal Emission S.E.M. (Zeiss DSM 960) $25 / hour Field Emission S.E.M. (Hitachi 4500) $30 / hour X-ray analysis with Field Emission S.E.M. (Hitachi 4500) $35 / hour Polaroid 4" x 5" (Type 52 or 55) $3.00 / ea. Video Print (SEM) Video Print (SEM)   LM L.M. (Zeiss Axioplan 2) $7.50 / hour Video Print (LM) $4.00 / ea. Information on ordering supplies, protocols for specimen preparation, and other technical information can be obtained from the staff at CIMC (607-253-3804). Cornell Integrated Microscopy Center (CIMC) *e-mail: cimc-mailbox@cornell.edu phone: 607-253-3804 fax: 607-253-3803 *We prefer people contact us by e-mail.

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