Low Temperature examples

Frozen-hydrated leaf surface of tomato leaf viewed at -180 C without any coating. Scale Bar-100 micrometers. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.

Frozen-hydrated leaf glandular trichomes of tomato leaf viewed at -180 C without any coating. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.

High Pressure Frozen Brassica (Canola plant) ovule cells fractured at -120 C, coated with platinum and viewed frozen-hydrated at -180 C. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.

Yeast cells frozen, fractured and coated immediately with platinum at -160 C and viewed frozen-hydrated at -180 C. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.

Frozen-hydrated image of Dunaliella fractured at -170 C, sputter-coated with platinum and viewed at -175 C in the SEM. Fractured nucleus (N), chloroplast (C) and golgi (G) can be seen in addition to other organelles. Scale - 2 microns. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.

TEM: BioG 403 | Freeze Sub | Immunogold | Neg. Staining | Replica | Freeze-Fracture | High Pressure

SEM: BioG 401 | Low kV | Low Temp | X-Ray Map

Light: DIC | Flourescence Microscopy

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	Frozen-hydrated leaf surface of tomato leaf viewed at -180 C without any coating. Scale Bar-100 micrometers. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.
	Frozen-hydrated leaf glandular trichomes of tomato leaf viewed at -180 C without any coating. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.
	High Pressure Frozen Brassica (Canola plant) ovule cells fractured at -120 C, coated with platinum and viewed frozen-hydrated at -180 C. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.
	Yeast cells frozen, fractured and coated immediately with platinum at -160 C and viewed frozen-hydrated at -180 C. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.
	Frozen-hydrated image of Dunaliella fractured at -170 C, sputter-coated with platinum and viewed at -175 C in the SEM. Fractured nucleus (N), chloroplast (C) and golgi (G) can be seen in addition to other organelles. Scale - 2 microns. Hitachi S4500 FESEM/BALTEC Scanning Cryo Unit SCE 020.